The Central Dogma of Molecular Biology
Instructor: Mete Mülazımoğlu - UAA'19
Cell/Cellular Molecular Biology, Johns Hopkins University in Maryland - USA
Cell/Cellular Molecular Biology, Johns Hopkins University in Maryland - USA
In this course, you will learn more about the central dogma of molecular biology: From DNA to protein. You might have learned the two steps of central dogma, transcription and translation, but let me tell you something: they are not that simple. In this course, you will discover the untold world of protein synthesis where there is more and detailed information. You will understand how the transcription really begins, what are cofactors, how does our cells regulate translation and so forth. But make no mistake: this information brings a huge amount of unknown with it. Even though you will learn more, you will be asking more as well. Besides the facts, I will be teaching you experiments like EMSA, ChIP and ribosome profiling. This is a 6-week course, one 1-hour class per week. There will be regular assignments after each class and two main assignments.
Course Goal:
Gain a better understanding of transcription and translation
Learn real-life experiments that scientists use all the time
Week 1 – Synthesis of pre-mature RNA
Week 2 – Cofactors, enhancers and how to detect them (EMSA, ChIP, and enhancer reporter)
Week 3 – Pre-mature RNA, mature RNA (RNA processing)
Week 4 – tRNAs, rRNAs, and Codon table
Week 5 – Translation and kinetic proofreading
Week 6 – Open reading frames, regulation of translation, and post-transcriptional modifications